This review is destined for a comprehensive assessment of the phytochemistry and medicinal properties of Tecoma stans, a widely used plant in folk cultures, as a traditionally safe and effective treatment for different diseases and complications. The attainable and reachable sources of T. stans confirmed its origin, ethnopharmacological properties, and therapeutic medicinal uses. Besides a hundred chemical compounds that have been isolated, the main active constituents are flavonoids, alkaloids, phenolic acids, and fatty acids. T. stans exerted many medicinal benefits, including antidiabetic, anti-inflammatory, anti-cancer, antimicrobial, antioxidant, hepatoprotective, cardioprotective, and nephroprotective properties. However, there is a shortage of in vivo studies, especially adequate dosage and toxicity studies. More studies should be carried out for nutritional data. This review represents a scientific understanding of clinical correlations and applications of phytocompounds from T. stans in protecting and treating many complaints and disorders.

Introduction: Essential oils are considered a potential alternative to synthetic drugs in the management of diseases such as peptic ulcer (PU) and ulcerative colitis (UC). This study is concerned with comparing the therapeutic effects of Cuminum cyminum L, Carum carvi L, and Thymus vulgaris L. essential oils on PU and UC models induced by ethanol. Methods: Rats were divided into 10 groups; control groups were treated with saline and experimental groups with 500 mg/kg body weight of C. cyminum, C. carvi, or T. vulgaris essential oil. Curative effects were determined by measuring tissue oxidative markers, such as reduced glutathione (GSH), malondialdehyde (MDA), and myeloperoxidase (MPO), as well as the inflammatory marker prostaglandin E2 (PGE2), stomach pepsin (PEP), and colon alkaline phosphatase (ALP). Biochemical and histological examinations were done on stomach and colon tissues. Results: The current study proved the anti-ulcer effects of C. cyminum, C. carvi, and T. vulgaris essential oils. They improved the oxidative and inflammatory markers in both stomach and colon tissues and modulated stomach PEP and colon ALP activities. T. vulgaris essential oil modulated GSH and MDA levels resulting in a significant elevation in GSH levels by 120.43% and 99.46% and a significant reduction in MDA levels by 20.05% and 24.1% in PU and UC models, respectively. C. carvi essential oil was the most effective in restoring PGE2 by 71.51% compared to UC group. Results were confirmed by the morphological and histopathological changes. Conclusion: C. cyminum, C. carvi, or T. vulgaris essential oils might be used in the management of acute PU and UC.

Introduction: Gentamicin (Gen) causes renal toxicity by inhibiting protein synthesis in kidney cells, causing proximal tubule cell necrosis and renal failure. Herein, we examined the nephroprotective effect of date palm seed extract (DPSE) and one herbal mixture (HM; composed of Tribulus terrestris, Aerva lanata, Andrographis paniculata, and Raphanus sativus) against Gen-induced renal toxicity in mice with special reference to the possible role of retinoid X receptor alpha (RXR-α) and protease-activated receptor 2 (PAR-2) in this effect. Methods: Thirty-two male Balb/c mice divided randomly into four groups were either treated with saline, Gen (225 mg/kg/i.p., daily from day 3 to day 10), Gen (225 mg/kg i.p.) daily from day 3 to day 10 and DPSE (100 mg/kg/p.o.) daily for 10 days, or Gen (225 mg/kg i.p.) daily from day 3 to day 10 and HM (100 mg/kg/p.o., daily for 10 days). Mice were sacrificed 24 hours after the last dose administration, and kidney tissues were dissected out, weighed, and subjected to histological, immunofluorescence, and biochemical assays. Results: The Gen-induced renal toxicity group demonstrated a significant decrease in RXR-α and a significant increase in PAR-2 protein expression. Treatment with DPSE or HM significantly improved Gen-induced effects on serum creatinine, blood urea nitrogen (BUN), white blood cells (WBCs), platelets, RXR-α extracellular matrix deposition, and PAR-2. Conclusion: The present study stated the nephroprotective effects of DPSE and HM and revealed, for the first time, the involvement of retinoid receptors and PAR-2 in Gen-induced renal toxicity as well as in the protective effects of the two tested natural products.

Introduction: Echinacea purpurea is a flowering plant commonly used as an herbal medicine despite insufficient scientific bases to validate its usage. The present study aimed to examine in vitro and in vivo hepatoprotective effects of aqueous and alcoholic extracts of E. purpurea flowers. Methods: In vitro protection against hepato-cytotoxicity was carried out on human HepG-2 cells using colorimetric tetrazolium (MTT) assay, while the in vivo hepatoprotective activity was studied against carbon-tetrachloride (CCl4) induced acute hepatotoxicity in rats. Results: The results revealed that the extracts of E. purpurea induced discernable in vitro protection on HepG-2 cells and in vivo against CCl4 induced hepatotoxicity. Both extracts were significantly able to restore the serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin, total protein, and albumin to normal levels compared to the CCl4 intoxicated group. In addition, the extracts markedly mitigated the oxidative stress by decreasing Malondialdehyde (MDA) and increasing superoxide dismutase (SOD) and glutathione (GSH) markers compared to the CCl4 intoxicated group. It was also associated with the down-regulation of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels in liver tissues. Histopathological examination revealed a decrease in hepatocytes’ degenerative changes and noticeable improvement of the liver damage by extracts of E. purpurea. Conclusion: These findings have proven that aqueous and alcoholic extracts of E. purpurea flowers have a significant hepatoprotective effect, probably owing to antioxidant, anti-inflammatory activities, and regulating apoptotic-related genes. This confirms the ethnomedicinal uses of E. purpurea in patients suffering from liver diseases.

Introduction: Non-communicable diseases are a cluster of metabolic diseases, which include type-2 diabetes, cancer, and cardiovascular diseases (CVDs). The aim of the current research was to incorporate dietary fibers (mucilage) and phytosterol for enriching chia seeds oil for producing new dietary supplements for cardio-protection from oxidative stress, inflammation, and dyslipidemia. Methods: Fatty acids profile, phytosterols, and phenolic compounds content of the prepared dietary supplement were assessed. The cardioprotective potency of the dietary supplement was evaluated in rats fed on a high-fat diet for a month. Biochemical parameters related to inflammation, oxidative stress, lipid profile, cardiac enzymes, and kidney function were determined in all rats. Results: The results revealed that dietary supplement was rich in omega-3 fatty acids. Beta-sitosterol and campesterol were the major phytosterols in chia seeds oil dietary supplement. Phenolic compounds were present by 25.9 ± 1.202 mg gallic acid equivalent (GAE)/g dietary supplements. Rats fed on the high-fat diet showed significant elevation (P < 0.05) in inflammatory markers, oxidative stress, dyslipidemia, and cardiac enzymes in association with the elevation of kidney function compared with normal rats. Administration of both doses of dietary supplement significantly (P < 0.05) improved all the studied biochemical parameters. The high dose of the dietary supplement was promising in the reduction of inflammatory markers, oxidative stress, and improved dyslipidemia in accordance with the reduction of all cardiac enzymes and kidney function. Conclusion: Dietary supplements investigated in the current research showed cardioprotective potency through its anti-inflammatory and dyslipidemic activities, which may be attributed to the presence of phenolic compounds, omega-3 fatty acids, phytosterols, and soluble dietary fibers.

Introduction: Parkinson’s disease (PD) is a neurodegenerative disease with a prevalence of 1% in the elderly worldwide. The aim of the research is to study the interrelationship of iron status, the immune system including inflammatory cytokines, brain divalent metal transporter 1 (DMT1), and dopamine receptors D1 (DRD1) in a PD rat model. The potential protective effects of grape seed and green coffee bean ethanol extracts and quercetin were also studied. Methods: Phenolic and flavonoid contents of grape seed and green coffee bean and in vitro free radicals scavenging activities of the extracts and quercetin were determined. Male rats were divided into five groups. Group 1 served as normal control (NC), group 2 represented Parkinsonian control (PC). Groups 3, 4, and 5 were the test groups treated by daily oral green coffee bean, grape seed extracts, and quercetin, respectively. PD was induced by rotenone in groups 2 to 5. Brain oxidative stress, DMT1, and DRD1 expressions, and histopathology were assessed. Parameters of the immune system, represented by plasma interferon-gamma (IFNγ) and CD4, and brain tumor necrosis factor-alpha (TNF-α) along with iron status were also determined. Results: Phenolic and flavonoid contents of green coffee bean were high compared to grape seed (P < 0.05). Quercetin experienced the highest in-vitro free radicals scavenging activities. Iron deficiency anemia, together with elevated IFNγ, TNF-α, DMT1 expressions, and brain malondialdehyde (MDA) were demonstrated in PC compared to NC (P < 0.05). Also, reduction in CD4 and brain reduced-glutathione (GSH) (P < 0.05) were noticed in PC with brain histopathological alterations. Different treatments showed variable improvements in the majority of parameters (P < 0.05) and brain histopathology. Conclusion: Iron deficiency anemia might result from cytokine elevation in PD. Reduced DRD1 and altered immune system including cytokines together with increased brain DMT1 might induce neurodegeneration in PD. Different treatments showed variable neuroprotective effects through modulation of inflammation, oxidative stress, immune system, iron status, DMT1, and DRD1.

Introduction: Recently, the recovery of waste products from plants as a source of biologically active compounds has increased interest. Therefore, the current research aims to evaluate the anti-inflammatory, immunomodulatory and antimicrobial activities of the fixed oil of Cucumis melo L seeds, as well as to investigate its physicochemical parameters and chemical composition. Methods: Anti-inflammatory activity was examined using carrageenan-induced rat paw edema assay. The antimicrobial activity was assayed against Staphylococcus aureus, Micrococcus luteus, Enterococcus faecalis, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Candida albicans by well diffusion method. The chemical composition of the oil was determined by gas chromatography/mass spectrometry (GC/MS), α-tocopherol was estimated by high-performance liquid chromatography (HPLC). Results: Cucumis melo oil had no toxicity and possessed a promising anti-inflammatory activity. Moreover, the oil exhibited a reasonable decrease in the pro-inflammatory cytokines, including interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-α), and a significant increase in the anti-inflammatory cytokine (IL-10). The oil exhibited a reasonable antimicrobial activity against all tested organisms. The major identified compound in the unsaponifiable matter was (1-methyldodecyl) benzene (8.76%), while the major fatty acid was methyl linoleate (14.10%). The results of physicochemical characterization revealed the better quality of Cucumis melo oil. The amount of α-tocopherol in the oil was 23.5 μg/mL, which is considered a reasonable amount. Conclusion: These findings indicate that the fixed oil of Cucumis melo L seeds might be used as a safe natural anti-inflammatory, immunomodulatory and antimicrobial agent.

Introduction: Hymenosporum flavum (Hook.) F. Muell. is the sole species within the genus Hymenosporum is known for its antimicrobial activity. The current study aims to examine the prospective activity of H. flavum as a safe supporter of sorafenib (as a reference standard) against hepatocellular carcinoma (HCC). Methods: Isolation and identification of compounds were made by chromatographic and spectroscopic methods. A fingerprint for the plant extract was done using HPLC-MS/MS spectrometric analysis. The total plant extract was examined in vitro for HCC activity. The isolated flavonoids were examined for their cytotoxic activities using molecular docking studies against both RAF-1 and ERK-2, and the promising compounds were further examined in vitro using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: Two new flavonols were isolated from the leaf extract of H. flavum (Hook.) F. Muell., quercetin-3-O-(glucopyranosyl 1→2 ribopyranoside) (1) and kaempferol-3-O-(glucopyranosyl 1→2 ribopyranoside) (2), accompanying other six known flavonoids (3-8), and identified via spectroscopic analysis. Moreover, HPLC- PDA/MS/MS spectrometric analysis revealed the presence of seventy phenolic metabolites. The cytotoxic activity of the plant extract confirmed its potential action on HepG2 cells indicated by the production level of lactate dehydrogenase (LDH) upon treatment compared with the normal cells. The isolated flavonoids were examined for their cytotoxic activity using molecular docking studies against both RAF-1 and ERK-2 as proposed mechanisms of their anticancer activities. Furthermore, compounds 1 and 3, which showed the best in silico results, were further examined in vitro using qRT-PCR. They exhibited promising inhibitory activities against both RAF-1 and ERK-2 gene expression. Moreover, they showed promising cytotoxic activities indicated by the MTT assay. Also, both of them improved the efficiency of sorafenib in targeting both RAF-1 and ERK-2 pathways suggesting synergistic combinations. Conclusion: Our findings showed the potential cytotoxic activity of H. flavum extract on HepG2 cells. Some isolated compounds (1 & 3) exhibited promising inhibitory activities against both RAF-1 and ERK-2 gene expression giving a lead future study for these compounds to be used in pharmaceutical preparations either alone or in combination with sorafenib.

Introduction: Alzheimer’s disease (AD) is a neurodegenerative problem that is increased progressively due to the increment of aging worldwide. Phytochemicals play an important role in the protection from neurodegeneration. The present study aimed to evaluate the protective effect of two dietary supplements (DS) rich in betalains, anthocyanins, and omega-3 fatty acids against AD. Methods: Two dietary supplements (DS I and DS II) were prepared; the first one was a mixture of anthocyanin-rich extract of purple carrot and flaxseed oil (DS I), while the second was a mixture of betalains-rich extract of beetroot and flaxseed oil (DS II). The protective effects of both DS were evaluated in an AD model. AD was induced in mice by intracerebroventricular (ICV) injection of streptozotocin (STZ) (3 mg/kg). Biochemical changes in brain tissue and plasma were determined. Behavioral of mice was evaluated through Y–maze test, Morris water maze, and novel object recognition test. Changes in brain tissues were assessed through histopathological examination. In vitro antioxidant activities of DS I and DS II were evaluated. Also, the contents of total phenolics, anthocyanins, betalains, and fatty acids profile were assessed. Results: Both DS investigated in the present study showed significant improvement (P < 0.05) in acetylcholinesterase, antioxidant enzymes, tumor necrosis factor-α (TNF-α) and malondialdehyde (MDA)in brain tissue and butyrylcholinesterase in plasma in association with amelioration in the behavioral tests and histopathological changes of the brain tissue. Conclusion: Both DS showed protective effects against STZ induced AD in mice due to the presence of anthocyanins, betalains, and omega-3 fatty acids.

Introduction: Garlic has many pharmacological properties such as antibacterial, anti-inflammatory, and antioxidant activities. This study aimed to investigate the possible mechanisms of the hepatoprotective effect of white garlic extract (WGE) and black garlic extract (BGE) against preneoplastic lesions induced by N-nitrosodiethylamine (NDEA) in rats. Methods: Forty-two rats were randomly distributed into six equal groups. Oral administration of WGE and BGE began 3 weeks before injection of NDEA. Group 1 was kept as the negative control, while the other groups were injected by a single intraperitoneal dose of NDEA in the 3rd week, followed by 2 subcutaneous injections/week of CCl4 till six weeks to induce preneoplastic lesions. Group 2 kept positive control and groups 3, 4, 5, and 6 were given WGE and BGE each of them at 250 and 500 mg/kg, respectively, for six weeks from the beginning. Serum liver enzymes, total protein, albumin (Alb), total bilirubin (TBil), and antioxidant enzymes were measured. Malondialdehyde (MDA), glutathione (GSH), tumor biomarkers, along with the content of 8-hydroxy-2-deoxyguanosine (8-OHdG) in liver DNA were estimated and liver histopathology was performed. Results: WGE and BGE significantly decreased the serum liver enzymes, TBil, tumor biomarkers, lipid peroxidation but increased total protein levels. The extracts significantly increased antioxidant enzymes, decreased 8-OHdG content in liver DNA, and alleviated histopathological lesions in the liver. Conclusion: The results affirm the hepatoprotective effect of WGE and BGE against NDEA-induced preneoplastic lesions in rats. This effect may be due to inhibition of lipid peroxidation, reduction of tumor biomarkers, enhancement of antioxidant enzymes, or reduction of 8-OHdG content in liver DNA.

Introduction: In the present research, the health benefits of the traditional Egyptian food called Kishk Sa′eedi (KS) and KS mixed with gum Arabic (GA) or with a mixture of GA and pomegranate seed oil (PSO) were studied in a rat model of metabolic syndrome (MS) induced by feeding high fructose high hydrogenated fat diet (HFFD). Methods: Rats were divided into a normal control group (NC) fed on a balanced diet (Diet 1), a MS control (MSC) receiving HFFD (Diet 2), and three test groups feeding on HFFD containing KS (Diet 3), KS with GA (Diet 4), and KS with GA and PSO (Diet 5), respectively for five weeks. Biochemical and histopathological changes were assessed. Results: Significant increase in blood glucose, plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST), urea, creatinine, uric acid, malondialdehyde (MDA), dyslipidemia and reduction in reduced glutathione (GSH) were demonstrated in MSC compared to NC (P < 0.05). Significant elevation in liver fat, MDA and gene expression of interleukin-6 (IL-6) with significant down-regulation of peroxisome proliferator-activated receptor (PPAR-α) were noticed in MSC compared to NC (P < 0.05). The three test diets improved plasma high-density lipoprotein-cholesterol (HDL-C), uric acid, MDA, liver PPAR-α and IL-6 expression (P < 0.05) compared to MSC without affecting liver lipids. Blood glucose, plasma dyslipidemia, AST, creatinine and urea were improved by diet 3 and diet 5 (P < 0.05). Diet 3 elevated GSH and reduced ALT and MDA (P < 0.05). Histopathological changes induced by HFFD in both liver and kidney showed variable improvement by feeding the tested diets. Conclusion: The tested diets significantly improved MS rat model with superiority to diet 3.

Introduction:Nutraceuticals might serve as protective agent against liver cancer induced by pro-cancerous chemicals that initiate high oxidative stress, inflammation and affect DNA integrity. The aim of the present research was to study the prevention of hepatocellular carcinoma initiation induced by N-nitrosodiethylamine (NDEA) through treatment by nutraceuticals.Methods:Two nutraceuticals were prepared; the first (NI) was a mixture of different extracts of green tea, wheat germ and tomato, the second one (NII) was composed of extracts mixture of broccoli, hazelnuts and carrot. Total flavonoids and flavonols were determined in the nutraceuticals. Four groups of rats were run; the first served as control normal, the other three groups were treated by intraperitoneal injection of NDEA, one of these groups was designated as control NDEA, the other two groups (test groups) were treated daily with oral doses of NI and NII, respectively. The experiment continued for 8 weeks. Plasma transaminases, alkaline phosphatase and catalase activities, total protein, albumin, malondialdehyde (MDA) and tumor necrosis factor-α (TNF-α) along with liver MDA level and catalase activity were assessed.Results:NI showed higher flavonoids and lower flavonols than NII (P< 0.05). High oxidative stress and inflammation biomarkers, liver dysfunction, reduced plasma albumin and total protein were demonstrated in control NDEA compared to control normal (P< 0.05). Test groups showed significant improvement in all parameters (P< 0.05) compared to NDEA control. NI was superior in improving plasma transaminases and catalase activities, MDA and TNF-α levels and liver catalase activity compared to NII (P< 0.05).Conclusion:Both NI and NII might prevent liver cancer initiation during exposure to carcinogenic agents, NI being superior to NII.

Introduction: Duqqa is a condiment, consisting of black pepper, cumin, sesame, coriander and high amount of salt. Reducing salt and adding other beneficial items to traditional duqqa can make it suitable dietary supplement for diabetes management. The current study aimed to assess the effect of a modified duqqa on diabetes and its complications in diabetic rats. Methods: The modified duqqa was formulated by mixing grounded fermented wheat, sesame, coriander, cumin, chicory leaves, cinnamon, turmeric and date seeds powder and studied in diabetic rats which were developed by streptozotocin-nicotinamide injection. Thirty-two rats were divided into four groups (n = 8) including non-diabetic, diabetic control and the other two groups fed on balanced diet supplemented with either 10 or 20% of duqqa prior the induction of diabetes (for one week) to the end of the experiment (8 weeks). Results: The dietary supplementation with 10 and 20% of the formulated duqqa prior the induction of diabetes did not delay the onset of diabetes in rats but produced reduction (32.56% and 50.47%, respectively) in the glucose levels of diabetic rats. Also, diabetic rats fed on the formulated duqqa showed insulin concentrations higher than that of diabetic control rats. Feeding diabetic rats on the formulated duqqa reversed the elevation of kidney lipid peroxidation and nitric oxide, limited the disturbance in the lipid profile as well as liver and kidney functions and elevated both serum and femur magnesium concentrations. Conclusion: The results indicated the hypoglycemic effect of the formulated duqqa and its efficiency in delaying diabetes complications.

Introduction: Polysaccharides have numerous therapeutic values including immunity stimulation, angiotensin converting enzyme inhibition, wound healing, anti-diabetic and cytotoxic activities, in addition to their potent anti-oxidant properties. This work examined the gastroprotective and ulcer healing potential of mucilage fraction isolated from Solenostemma argel (Delile) Hayne (MFA) against ethanol-induced gastric ulcer in rats. Methods: Forty Sprague-Dawley rats were divided into five groups (n=8); normal control (I), ethanol control (II), 20 mg/kg Antodine pretreated rats (III), 100 mg/kg, and 200 mg/kg MFA pretreated rats, respectively (IV & V). All rats in groups II-V received single intragastric dose of ethanol (5 mL/kg) to induce gastric ulcer. Gastric mucosal injuries were assessed by stomach gross examination as well as histopathology, and immunohistochemistry of apoptotic markers. Also, several biochemical parameters including oxidative stress, proinflammatory cytokines, cytoprotective and cell proliferative biomarkers were measured. Results: High-performance liquid chromatography (HPLC) analysis of MFA revealed its composition of glucose, D-fucose and N-acetyl glucosamine as monosaccharaides, in addition to glucuronic and galacturonic acids. The data showed that MFA, 200 mg/kg had potent antioxidant, anti-inflammatory, cytoprotective, cell proliferative, and antiapoptotic activities which were better than Antodine. Conclusion: This study revealed that MFA had significant gastroprotective effects against ethanol-induced gastric injuries and could be a promising adjuvant therapy for ulcer treatment.

Introduction: Rumex spp. have been used in folk medicine either as food or as medicine for the treatment of several diseases including constipation, fever, inflammation, bacterial and fungal infections. This study aimed to evaluate the anti-inflammatory and antimicrobial activities of the successive extracts of the aerial parts of Rumex pictus Forssk. growing in Egypt, and to identify the chemical constituents in the bioactive extract. Methods: Ether, chloroformic, and 70% methanolic extracts of the aerial parts of R. pictus were assayed for their in vivo anti-inflammatory activity using carrageenan-induced rat hind paw edema method. These extracts were also tested for their in vitro antibacterial and antifungal activities using disc diffusion method. Results: The 70% methanolic extract of R. pictus exhibited significant anti-inflammatory, antibacterial, and anti-candida activities. Thus, fractionation of the bioactive extract was performed which led to the isolation of three anthraquinones, as well as, seven flavonoids. Conclusion: Rumex pictus possesses anti-inflammatory and antimicrobial activities which reinforce its use in ethnomedicine.

Introduction: Steatohepatitis, which is the deposition of fat in the liver with inflammation and starting of necrosis, can induce cardiovascular diseases (CVDs). The aim of this research was to study the preventive effects of steatohepatitis and CVD by ethanol extract of two quinoa varieties (quinoa 1 and hualhuas) in rats. Methods: Phenolic and flavonoid compounds were determined in the extracts utilizing colorimetric and high-performance liquid chromatography techniques. The 2,2-diphenyl-1-picryl-hydrazil (DPPH) scavenging activity was assessed for the extracts. Rats were divided into four groups, the first group was fed on a balanced diet (negative control), and other groups consumed a high fructose-fat diet (HFFD) to induce steatohepatitis and CVD. The second group served as a positive control; however, the third and fourth groups were treated by ethanol extract of quinoa 1 and hualhuas, respectively. Different biochemical changes, as well as liver and heart histopathology, were followed. Results: Results showed significant elevation in liver lipids, plasma malondialdehyde, total cholesterol (T-C), triglycerides and low-density lipoprotein cholesterol with reduction of high-density lipoprotein cholesterol (HDL-C) and total antioxidant as well as a significant increase in T-C/HDL-C in control positive group (P < 0.05) compared to control negative group. Plasma parameters and liver lipids were improved by the extracts; hualhuas was superior concerning the effect on lipid while quinoa 1 was more efficient in reducing oxidative stress. The oral glucose tolerance curve and the histopathology of the liver and heart tissues were improved by both extracts. Total phenolic and DPPH scavenging activity were higher in quinoa 1 than hualhuas. Protocatechuic and rutin were the major identified phenolic acid and flavonoid compounds, respectively in the extracts. Conclusion: Quinoa extracts are able to prevent the progression of steatohepatitis and CVD, and might be beneficial in patients with such diseases.

Diabetes mellitus (DM) is considered as one of the most common metabolic disorders affecting huge number of people worldwide. Despite the availability of large numbers of drugs in the market to treat the disease, there is still a need for new sources to deal with the problem and avoid side effects. In the pursuit of discovering safer and more effective anti-diabetic drugs, herbal and folk medicine drugs from regions all over the world have captured researchers’ interest. Middle Eastern and North African medicinal plants contain a variety of pharmacologically active components that have shown to possess promising anti-diabetic potential. However, few data have been reported about medicinal plants from these regions in comparison to plants from other regions. Anti-diabetic medicinal plants from the MENA (the Middle East and North Africa) region, their role in controlling DM, and suggested mechanisms for the anti-diabetic activity of some medicinal plants are discussed in this review. Many of these plants have not been fully investigated and characterized, yet they have great potential for further development as anti-diabetic drugs.

Introduction: Apricot (Prunus armeniaca L.) has been widely used for the treatment of several disorders such as liver diseases, but the hepatoprotective and anticancer activities of its seeds were not studied before. In this study, we evaluated the pharmacological effects of apricot seeds extracts and amygdalin on prevention of liver damage and treatment of hepatocellular carcinoma.Methods: Amygdalin contents of apricot seeds in ethanolic extracts were determined using high performance liquid chromatography (HPLC) then, the ethanolic apricot seeds extract and amygdalin were evaluated for its hepatoprotective activity against carbon tetrachloride-induced hepatotoxicity and anticancer activity against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis. Results: The amount of amygdalin was 5.72 g and 10.22 g/100 g extract for 70% and 99.9% ethanolic apricot seeds extracts, respectively. Pretreatment of the rats with 70% and 99.9% ethanolic apricot seeds extracts (100 mg/kg), amygdalin and silymarin (50 mg/kg) prevented elevation in liver function parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) caused by carbon tetrachloride injection with significant increase in albumin, total proteins, and no effect on total direct bilirubin when compared to those in hepatotoxic group. Both extracts also showed anticancer activity against hepatocellular carcinoma via diminishing the elevated serum levels of AST, ALT, ALP, total, direct bilirubin, albumin, total proteins, alpha-fetoprotein, malondialdehyde (MDA) and nitric oxide (NO) and elevating the decreased hepatic reduced glutathione (GSH) level when compared with NDEA- intoxicated group.Conclusion: Apricot seeds possess hepatoprotective and anticancer activities that justify its traditional use, and its potential for the treatment of liver diseases including hepatocellular carcinoma.

Introduction: The ethyl acetate fraction of the Saudi Lavandula coronopifolia Poir has been previously reported to have hepatoprotective activity against ethanol-induced oxidative stress. The aim of the current study was to investigate the chemical composition, cytotoxic effect, and antioxidant activities of ethyl acetate fraction of the aerial parts of Saudi L. coronopifolia Poir. Methods: Air dried aerial parts of L. coronopifolia were extracted using 90% ethyl alcohol. The dried extract was suspended in water, defatted with light petroleum and then fractionated with ethyl acetate. The ethyl acetate fraction was subjected to ultra performance liquid chromatography-tandem mass spectrometeric (UPLC-ESI/MS/MS) analysis in a negative ionization mode. The antioxidant activity of the fraction was determined using free radical 2,2-diphyenyl-picrylhydrazyl (DPPH) scavenging assay and its cytotoxic effect against HepG2 (human hepatocarcinoma) and MCF-7 (human breast carcinoma) cells were determined using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium (MTT) cell viability assay. Results: The major components of the ethyl acetate fraction included carvacrol-O-diglucoside, (34.98%) and trihydroxy ursolic acid (12.07%). Moreover, the DPPH radical scavenging activity of ethyl acetate fraction was measured. The ethyl acetate fraction revealed an antioxidant potential with EC50 17.8 ± 1.3 µg/mL. Additionally, he ethyl acetate fraction showed cytotoxic activity against HepG-2 and MCF-7 cells with IC50=29.3 ± 0.9 µg/mL and 14.6 ± 0.3 µg/mL, respectively. Conclusion: The ethyl acetate fraction of the Saudi L. coronopifolia has antioxidant activity and also cytotoxic activity against breast and liver cancer cells.

Introduction: The purpose of this study was to evaluate the phytoconstituents and various bioactivities of Pleiogynium timorense bark as a step towards the production of a new drug from natural origin to overcome the complications of the synthetic drugs. Methods: The phenolic compounds were isolated and identified by chromatographic and spectroscopic methods as ultraviolet (UV) and nuclear magnetic resonance (NMR) spectra. The isolated compounds, as well as 70% methanol extract of P. timorense bark were tested for cytotoxicity against human colon carcinoma (HCT 116), human hepatocellular liver carcinoma (HepG2), normal melanocytes (HFB-4) and human breast carcinoma (MCF-7) cell lines. In addition, the methanol extract was evaluated for renal protective, hepatoprotective, antioxidant and antihyperglycaemic activities. Results: Seven phenolic compounds were isolated from the bark of the plant for the first time which were identified as; pyrogallol, catechin, gallic acid, kaempferol, quercetin, rutin and quercetrin. Moreover, the methanol extract of the bark showed a promising cytotoxic effect against HepG2 cell line more than that of the isolated compounds comparing with doxorubicin (a positive control), where catechin and gallic acid showed moderate effects. In addition, the methanol extract showed potent antioxidant, hepatorenal protective and antihyperglycaemic effects. Conclusion: Pleiogynium timorense extract possesses a potent cytotoxic effect against HepG2 cell line and significant antioxidant, hepatorenal protective and antihyperglycaemic effects.

Introduction: The interrelation between cardiovascular diseases (CVDs) and renal dysfunction and the beneficial role of nutraceuticals are worthy to be studied. Nutraceuticals with anticancer effects are gaining great importance. The aim of this research was studying the anti-cancer, CVDs prevention and renal dysfunction properties of γ-oryzanol (γ-O) and rice bran oil/γ-O mixture (RBO/γ-O) as nutraceuticals. Methods: Rats were divided into 7 groups. Group 1 was fed on balanced diet and served as normal control (NC). Group 2 consumed high-fat-sucrose diet (HFSD) as CVD control. Groups 3 and 4 were fed on HFSD and treated by γ-O and RBO/γ-O, respectively. Group 5 was maintained on HFSD with cisplatin injection (cardiorenal syndrome control) (CRSC). Groups 6 and 7 were treated like group 5 and given either γ-O or RBO/γ-O. Plasma lipid profile, malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), catalase activity, creatinine and urea were determined besides urinary creatinine clearance. Nutraceuticals’ anticancer effect was assessed in hepatocellular carcinoma cell (HepG2) line. Results: Significant increases (P < 0.05) in lipid parameters with reduction of high-density lipoprotein cholesterol (HDL-C) were noticed in CVD control compared to NC group; the same changes were demonstrated in CRSC with lesser extent. In CVD control and CRSC groups; a significant increase (P < 0.05) in MDA and TNF-α with a reduction in catalase were noticed. Kidney dysfunction was demonstrated in the CRSC group. Administration of both RBO/γ-O and γ-O produced variable improvements in all parameters in both models and had anticancer effects. Conclusion: RBO/γ-O and γ-O had protective effects on CVDs and cardiorenal syndrome as well as anti-hepatocellular carcinoma activities with superiority of RBO/γ-O.

Introduction: Achillea fragrantissima extract has been used in folk medicine as an anticancer. The present study describes the antitumor activities of the extract and the flavonoid compounds and attempts to explain the mechanisms underlying these activities. Methods: The whole plant of A. fragrantissima was collected and ground to produce a fine powder which subjected to the maceration process. Step gradient elution was used using silica gel vacuum liquid chromatography (VLC). The fractions were compared by thin layer chromatography (TLC) and concentrated. Final purification was performed using Sephadex LH-20 to give three compounds cirsiliol, chrysosplenol D and cirsimaritin from F4 and one compound, eupatilin-7-methyl ether from F6. In this study, Ehrlich’s ascites carcinoma (EAC) model was used as the model of cancer. Results: Achillea fragrantissima extract and its isolated methoxylated flavonoids significantly reduced the weight of tumor discs compared to EAC-control group. In addition, cirsimaritin and eupatilin 7-methyl ether treatments produced a dose-dependent reduction in tumor weight. Serum tumor necrosis factor-alpha (TNF-ɑ) level showed that A. fragrantissima extract and its isolated methoxylated flavonoid compounds significantly reduced its serum level compared to the EAC-control group. Furthermore, A. fragrantissima extract and the flavonoids significantly increased the Total antioxidant capacity (TAC) compared to EAC-control. A. fragrantissima extract and its isolated methoxylated flavonoids produced a better differentiation of tumor cells, with reduced nuclear pleomorphism and better formed tubular structures especially with high dose indicating the induction of apoptotic mechanism. Conclusion: Achillea fragrantissima extract and its isolated methoxylated flavonoids exhibit antitumor activities that may be attributed to the antioxidant properties and the induction of the apoptotic process.

Introduction: Protection of brain against accelerated aging helps avoiding the occurrence of neurodegenerative diseases. So, the current work was conducted to evaluate the rescuing role of kumquat fruits crude ethanol extract, carrot seeds ethanol and petroleum ether extracts against the brain aging induced by D-galactose in rats. Methods: Forty male Sprague Dawley rats were divided equally into five groups. Group I was served as normal control, rats of group II were daily injected intraperitoneally (i.p.) with 150 mg/kg BW of D-galactose. Rats of group III, IV and V were daily injected i.p. with the same dose of D-galactose and administered orally with 250 mg/kg BW/day of kumquat fruits crude ethanol extract, carrot seeds ethanol extract and carrot seeds petroleum ether extract, respectively. After 6 weeks the rats were scarified, brain tissues were analyzed for malondialdehyde (MDA), catalase (CAT) as well as histological examination. Also, the plasma was analyzed for MDA, tumor necrosis factor-α (TNF-α), creatinine and urea levels, as well as CAT, butyrylcholinesterase (BChE), aspartate transaminase (AST) and alanine transaminase (ALT) activities. Results: From the results, it was elucidated that the tested extracts suppressed both the reduction in CAT and the elevation in MDA either in brain or plasma and the increase in plasma TNF-α, BChE as well as liver and kidney parameters. Conclusion: The tested extracts can be served as potent protective agents against the accelerated aging parameters which may be due to anti-oxidant and anti-inflammatory activities.

Introduction: Protection of liver from the aggressive force of various environmental and chemical agents is very important for the overall health of an individual. So, the present study aimed to evaluate the protection efficiency of crude extracts of red radish seeds and roots against paracetamol mediated hepatotoxicity in rats. Methods: Twenty-four male Wistar rats were divided into four groups. Group I was served as normal rats, Group II received orally single dose of 2 g paracetamol/kg body weight on the 22nd day, Group III and Group IV were administered orally with 300 mg/kg/d crude ethanol extract of either seeds or roots of red radish for 21 days, then received paracetamol on 22nd day. After 48 hours of paracetamol administration blood was withdrawn to determine the activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and Gama-GT (γ-GT) as well as total and direct bilirubin. Also, liver tissues were separated to determine malondialdehyde (MDA) and nitric oxide (NO) as well as histological changes. Results: Pretreatment of rats with crude ethanol extract of either seeds or roots of red radish significantly (P ≤ 0.05) suppressed the elevations in serum activities of ALT, AST, ALP, γ- GT, total and direct bilirubin as well as liver MDA and NO levels. The results of histopathologic examinations were consistent with the biochemical results. Conclusion: Seeds and roots of red radish have a protection efficiency against paracetamol mediated oxidative damage and hepatotoxicity in rats.