Bovine tuberculosis is an important global zoonosis. The causative agent of the disease is Mycobacterium bovis, belonging to the Mycobacterium tuberculosis complex. This study aimed to identify vertical transmission of Mycobacterium bovis in tuberculin positive pregnant dairy cows referred to the slaughterhouse and commercial sperm used to inseminate them in Gonbad, Gorgan province, Iran, by PCR assay. From March to September 2016, one thousand and seven hundred artificially inseminated cows were subjected to the tuberculin test; then, tuberculin positive cows were referred to the Gonbad slaughterhouse. Samples containing milk, lymph node, placenta, foetal abomasal fluid, and amniotic fluid were prepared from all affected cows and stored at -80 °C until analysis. Bacterial DNA was extracted from all specimens by a commercial kit. Detection was performed by amplifying pncATB gene using special primers via the polymerase chain reaction method. Results of tuberculin test revealed 144 positive cases (8.47%). PCR results for 48 milk samples, 120 biopsy samples, 63 samples of foetuses showed 9, 93 and 43 positive cases, respectively which all belonged to tuberculin positive samples. No positive sperm samples were identified. It was concluded that the infection rate with M. bovis in Montbéliarde cattle was high and also, that vertical transmission was not seen.

Endotoxaemia is an inflammatory condition which happens due to the presence of outer cell wall layer of Gram-negative bacteria in blood circulation, containing lipopolysaccharide commonly known as endotoxin. This condition causes high mortality in affected animals and sheep are highly susceptible in this regard. Several researchers have emphasised the therapeutic regimens of endotoxaemia and its sequels in sheep. Furthermore, sheep are among the most commonly used animal species in experimental studies on endotoxaemia, and for the past five decades, ovine models have been employed to evaluate different aspects of endotoxaemia. Currently, there are several studies on experimentally induced endotoxaemia in sheep, and information regarding novel therapeutic protocols in this species contributes to better understanding and treating the condition. This review aims to specifically introduce various treatment methods of endotoxaemia in sheep.

Lactic acid bacteria (LAB) may contribute to the food safety. In the present study, the antagonistic activity of 45 Bulgarian Lactobacillus homo- and heterofermentative strains of human and dairy origin, and 4 multibacterial formulas against Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) was assessed. In vitro tests were performed in different model systems - milk, soymilk, De Man Rogose Sharp (MRS) medium, to simulate real conditions in the food chain. The highest antagonistic activity was observed with cell-free supernatants of exponential MRS broth cultures of the strains isolated from breast milk, followed by lactobacilli from white brined and green cheese. The detected antimicrobial activity against the pathogen was strain-specific and depended on the culture conditions. Lactobacillus (reclassified as Lactoplantibacillus) plantarum strains, cultivated in skimmed milk and whey protein medium, were able to inhibit S. Typhimurium growth, while a limited inhibitory activity was detected for fermented soymilks. A bacteriocinogenic Ligilactobacillus (the previous Lactobacillus) salivarius strain reduced the number of living pathogenic cells during co-cultivation in whole milk. The inhibition was significant only when L. salivarius was inoculated in predominance. In case of underrepresented LAB number, S. Typhimurium over-growth was observed. Eight lactobacilli in combination as a multibacterial co-culture expressed synergic antagonistic effect against Salmonella and were pre-selected as promising. Further characterisation of their active metabolites, however, is needed before their classification as bio-protective agents.

The aim of this review was to summarise the information about the cases of highly pathogenic avian influenza in Bulgaria during the last two decades. According to the EMPRES-I FAO data, from January 2006 to the end of 2019, 141 HPAI outbreaks have been registered in Bulgaria, with two identified virus serotypes (H5N1 and H5N8). The H5N1 outbreaks were reported in 2006, 2010 and 2015. Almost all H5N1 cases were observed in wild birds, with only one outbreak in domestic chickens in a backyard farm in 2015. From 2016 to the end of 2019, 132 outbreaks of HPAI identified as H5N8 were recorded: sixteen in wild birds and the other 116 - in domestic poultry. Large farms with fattening ducks used for foie gras production in four administrative districts (Plovdiv, Haskovo, Stara Zagora, Dobrich) were mainly affected.

Behavioural disorders, including feather pecking and cannibalism, are a common problem in both domestic and wild birds. The consequences of this behaviour on welfare of birds incur serious economic losses. Pecking behaviour in birds is either normal or injurious. The type of normal pecking behaviour includes non-aggressive feather pecking – allopreening and autopreening. Aggressive feather pecking aimed at maintenance and establishment of hierarchy in the flock is not associated to feathering damage. Injurious pecking causes damage of individual feathers and of feathering as a whole. Two clinical presentations of feather pecking are known in birds. The gentle feather pecking causes minimum damage; it is further divided into normal and stereotyped with bouts; it could however evolve into severe feather pecking manifested with severe pecking, pulling and removal, even consumption of feathers of the victim, which experiences pain. Severe feather pecking results in bleeding from feather follicle, deterioration of plumage and appearance of denuded areas on victim’s body. Prolonged feather pecking leads to tissue damage and consequently, cannibalism. The nume­rous clinical presentations of the latter include pecking of the back, abdomen, neck and wings. Vent pecking and abdominal pecking incur important losses especially during egg-laying. In young birds, pulling and pecking of toes of legs is encountered. All forms of cannibalistic pecking increase morta­lity rates in birds. Transition of various pecking types from one into another could be seen, while the difference between gentle, severe feather pecking and cannibalism is not always distinct.

Toxoplasma gondii and Neospora caninum, two obligatory intracellular protozoan parasites, are important causes of neonatal mortality and abortion in farmed ruminants worldwide. Previously, protozoan abortions in small ruminants were attributed to T. gondii, but the role of N. caninum in these abortions was uncertain. The aim of this study was to investigate the role of T. gondii and N. caninum in ovine abortion in Urmia, northwest of Iran using a molecular method. Overall, 130 placenta and brain samples of aborted ovine foetuses were collected. Extracted DNA from placenta and CNS tissues of the aborted foetuses were analysed using PCR with primers specific for T. gondii and N. cani­num. The association of the frequency of T. gondii and N. caninum infection of aborted foetuses with age and breed in flocks was also studied. The results showed that out of the 130 examined ovine foetuses, 5.3 and 2.3 % were PCR-positive for T. gondii and N. caninum DNA, respectively. In this study, no significant differences were recorded relating to Toxoplasma and Neospora infection in different age groups in flocks and among sheep breeds included in the present study (P>0.05). The results of this study proved the importance of T. gondii and N. caninum as reasons of abortion in the studied area.

The current study aimed to assess the ovarian and uterine haemodynamics in postpartum (pp) Egyptian buffaloes and their relation to steroid hormonal levels (progesterone and estrogen). Six multiparous buffalos with normal calving were examined by using trans-rectal Doppler sonography to assess the blood flow in ipsilateral and contralateral ovarian and uterine arteries. Doppler parameters viz. peak systolic velocity (PSV), blood flow volume (BFV), resistance (RI) and pulsatility index (PI) were measured at 1st till 6th postpartum weeks. In addition, diameters (mm) of the ovarian and uterine arteries and the vascularisation area (mm2) of ovary were determined. The blood samples were collected starting from 1st week post calving and every week thereafter following each ultrasound Doppler examination for assay of progesterone and estradiol. Results indicated that PI and RI increased consistently and significantly (P<0.05) till the 6th week post calving as compared to those recorded at 1st week in ipsilateral ovarian and uterine arteries. However, the blood flow volume and peak systolic velocity in both ovarian and uterine arteries decreased gradually from 1st week till the 6th week after calving. There were very strong positive correlations between PSV and BFV, in both ipsilateral and contralateral ovarian and uterine arteries. Blood concentrations of progesterone were lower (P<0.05) during the first four weeks than those recorded at 5th and 6th postpartum weeks. This trend was reversed for blood estradiol 17-β. The results of the present investigation concluded that Doppler ultrasonography is a suitable tool for assessing changes in ovarian and uterine perfusion during the puerperium.

Respiratory viral infections cause significant economic losses in sheep production. This preliminary molecular study aimed to detect the Maedi-Visna virus infection in Awassi sheep in three governorates in the middle region of Iraq. The presence of one or more of the specific four genes (gag, pol, env and LTR) were considered as positive result. A total of 210 blood samples of Awassi sheep were collected for the purpose of the project. The molecular prevalence of Maedi-Visna virus in sheep was 12.85% (27/210). As a result, Maedi-Visna virus was observed in sheep with chronic respiratory system disease with non-significant difference between governorates and between primers percentage (P>0.05). Sequencing studies strongly suggested that Maedi-Visna virus originated in Iraq. This is the first study describing Iraqi Maedi-Visna virus sequences with molecular characterisation of gag, pol, env and LTR genes, suggesting that Maedi-Visna virus originated in Iraq.

Trueperella pyogenes is associated with endometritis and metritis in cows. Traditionally these diseases are treated with antibiotics while new approaches include application of the mucolytic N-acetylcysteine. Therefore the study aimed to evaluate the sensitivity of clinical Trueperella pyogenes isolates (n=2) to oxytetracycline, doxycycline, N-acetylcysteine and their combinations. The potential for biofilm formation with/without tested drugs was investigated by the method of crystal violet staining. Minimum inhibitory concentrations (MIC) of oxytetracycline for T. pyogenes 1 and 2 were 16 and 64 µg/mL, respectively. MIC of doxycycline for both isolates was 32 µg/mL and for N-acetylcysteine - 8 mg/mL. Both Trueperella pyogenes isolates did not form biofilm. The growth of T. pyogenes 1 cultured in the presence of either oxytetracycline or doxycycline (0.0078–128 µg/mL) under conditions for biofilm formation was significantly inhibited at concentrations ≥ 1 μg/mL and 8 μg/mL, respectively. The growth of T. pyogenes 2 was not affected by the antibiotics. N-acetylcysteine at ≥ 4 mg/mL resulted in significant inhibition of the growth of both isolates and its combinations with the antibiotics did not inhibit their growth. The effect of N-acetylcysteine should be validated in clinical settings but its combinations with tetracyclines were not able to improve the sensitivity of T. pyogenes, isolated from cows with clinical metritis.

Sarcocystosis infection is caused by protozoan cysts of genus Sarcocystis spp. where S. hominis, S. heydorni (bovines) and S. suihominis (porcine) are the most relevant for humans because of their zoonotic potential. S. cruzi, S. suihominis and S. ovicanis represent the most pathogenic species for cattle, pigs and sheep respectively. This infection has a worldwide importance due to its high transmission; besides to represent a zoonosis, it generates great economics losses. The main diagnostic methods for this disease are artificial digestion, PCR, indirect ELISA, and compression analysis. It’s important to highlight few studies on Sarcocystis spp., especially the ones involving the pursuit of effective treatments to control the infection for both humans and animals, however, some studies have reported that treatments such as cotrimoxazole and albendazole with or without prednisone are effective in counteracting symptoms in humans, considering the lack of reports about Sarcocystis spp. prevalence in Colombia.

This study was aimed to describe some glycosylation changes in the Nurse cell of Trichinella spiralis in mouse skeletal muscle. Tissue specimens were subjected to lectin histochemistry with Maackia amurensis lectin (MAL), Peanut agglutinin (PNA) and neuraminidase desialylation in order to verify and analyse the structure of α-2,3-sialylated glycoproteins, discovered within the affected sarcoplasm. The expressions of two sialyltransferases were examined by immunohistochemistry. It was found out that the occupied portion of skeletal muscle cell responded with synthesis of presumable sialyl-T-antigen and α-2,3-sialyllactosamine structure, that remained accumulated during the time course of Nurse cell development. The enzymes β-galactoside-α-2,3-sialyltransferases 2 and 4, which could be responsible for the sialylation of each of these structures, were however not present in the invaded muscle portions, although their expressions in the healthy surrounding tissue remained persistent. Our results contribute to the progressive understanding about the amazing abilities of Trichinella spiralis to manipulate the genetic programme of its host.

Cancer cells heavily utilise angiogenesis process to increase vascularisation for tumour mass growth and spread, so targeting this process is important to create an effective therapy. The AMHA1 strain of Newcastle disease virus (NDV) is an RNA virus with natural oncotropism. NDV induces direct tumour cytolysis, apoptosis, and immune stimulation. This work aimed to test NDV anti-angiogenic activity in a breast cancer model. To evaluate NDV’s antitumour effect in vivo, NDV was tested against mammary adenocarcinoma AN3 transplanted in syngeneic immunocompetent mice. In vivo antiangiogenic activity was evaluated by quantifying the blood vessels in treated and control tumour sections. In vitro experiments that exposed AMN3 mammary adenocarcinoma cells and Hep-2 laryngeal carcinoma cells to NDV at different time intervals were performed to identify the exact mechanism of anti-angiogenesis by using angiogenesis microarray slides. In vivo results showed significant tumour regression and significant decrease in blood vessel formation in treated tumour sections. The in vitro microarray analysis of 14 different angiogenesis factors revealed that NDV downregulated angiopoietin-1, angiopoietin-2, and epidermal growth factor in mammary adenocarcinoma cells. However, NDV elicited a different effect on Hep-2 as represented by the downregulation of inducible protein 10, intracellular adhesion molecule-1, and basic fibroblast growth factor beta in NDV-infected tumour cells. It was found out that microarray analysis results helped interpret the in vivo data. The results suggested that the NDV oncolytic strain reduced angiogenesis by interfering with angiogenesis factors that might reduce tumour cell proliferation, infiltration, and invasion.

Doxycycline is a broad-spectrum tetracycline antibiotic widely used in veterinary medicine. The current review aims to summarise the available data about pharmacokinetics in mammalian species of veterinary interest and to indicate the basic strategies for refining dosage regimens in order to use this antibiotic reasonably. Additionally, the available data about population pharmacokinetics are reviewed as this approach exhibits a number of benefits in terms of determination of drug phar­ma­cokinetics, prediction of drug disposition and interpretation of the variations in the pharmacokinetic parameters. Further research with animal species of veterinary interest and pathogens causing diseases in animals is needed to clarify the pharmacokinetics and pharmacodynamics of doxycycline.

The present studies were carried out to establish the causes for appearance, prevalence, age- and sex-related features, the frequency and 24-hour pattern of clinical signs accompanying the tongue rolling stereotypy in cattle. The animals were distributed in 2 groups: group I (n=1055) – control animals that did not demonstrate the tongue play orosthenic syndrome and group II (n=48) – cattle manifesting this stereotypy. Both groups were uniform with regard to age, breed, body condition and sex. They were placed under equal housing conditions (animal hygiene parameters, feed quantity and quality) and activity regimens (walks, feeding etc.). It was found out that the causes for demonstration of the tongue rolling hyperactivity in cattle were the deprivation from food and boredom, due to deficiency of environmental stimuli. The studied stereotypy was observed in cattle in different age groups – suckling and weaned calves, as well as heifers, including pregnant ones. The tongue play activity was no longer exhibited by heifers after calving and moving into the basic herd of lactating cows. The highest intensity (up to 80%) of this orosthenic syndrome was manifested before the morning feeding, between 9.00 and 12.00 AM. Most commonly, the tongue rolling activity was found out in heifers between 13 to 25 months of age. The studied oral stereotypy was not accompanied with changes in measured clinical parameters – rectal body temperature, pulse and respiratory rates, rumen movements, as well as in other activities e.g. rumination, eructation, appetite, sentience, locomotion etc.

Avian orthoavulavirus, commonly known as Newcastle disease virus (NDV) has been a constant threat for the poultry industry of Iran for decades. Recently, a couple of preliminary studies on backyard and commercial chicken suggested that a major subgenotype circulating in Iran may be VII(L) subgenotype, which is now known as VII.1.1 according to the new classification system. The unique subgenotype was not reported from other parts of the world and was slightly (≥3%) different from the closest group that was VIId. The study was conducted between July 2018 and March 2019 to determine the exact NDV genotypes/subgenotypes circulating in Iranian broiler poultry farms; five-hundred and forty chickens were sampled from thirty-six broiler farms located in eighteen provinces of Iran. As other genotypes/subgenotypes such as XIII and VI.2 are circulating in neighbouring countries, border provinces were also sampled. The F gene of the NDV isolates was sequenced and phylogenetic analysis was conducted. All the isolates clustered under the VII.1.1 group. The evolutionary analysis also revealed that the distances were between 0.0 and 0.7% meaning that the Iranian NDV circulating in broiler farms were not only of VII.1.1 sub-genotype, but also genetically very identical, indicating that the routine control measures for ND in Iran were not able to prevent the circulating NDVs. Although stricter biosecurity measures have been really effective in developed countries, surveillance of NDV to determine the circulating genotypes might also help us to implement better vaccination strategies in the future.

Osteoarthritis, also known as degenerative joint disease (DJD), is defined as a progressive and permanent long-term deterioration of the cartilage surrounding the joints. There is no known cause for primary DJD. However, there are a wide variety of causes for secondary DJD, such as trauma, abnormal wear of joints and cartilage, or a congenital defect present at birth such as an improperly formed hip. One of the most popular methods used to biologically enhance healing in the fields of orthopaedic surgery and medicine includes the use of autologous blood products, namely, platelet rich plasma (PRP). Reports suggest that PRP, presumably containing high levels of platelet growth factors, may promote the recovery of the affected cartilage. This case series presents clinical and radiographic findings of three dogs with osteoarthritis of the elbow and knee joints. Pain score were assessed by CBPI (Canine Brief Pain Inventory). Treatment with three-fold intra-articular application of PRP, obtained by double centrifugation method, resulted in significant improvement in the function of the affected joint. Therefore, it could be concluded that PRP was clinically effective in the treatment of osteoarthritis in these three cases.

Despite the fact that H9N2 avian influenza virus (AIV) is considered a low-pathogenic agent, frequent outbreaks of this subtype have caused high mortality and economic losses in poultry farms around the world including Iran. Coinfection with a respiratory pathogen or environmental factors may explain the exacerbation of H9N2 AIV infection. In this study, the role of infectious bronchitis (IB) vaccines (H120 and 4/91) and Newcastle disease (ND) vaccines (B1 and LaSota) on experimental H9N2 AIV infection was investigated in 180 broiler chickens allotted into 6 groups (n=30). At the age of 18 days, groups 3 and 4 received H120 and 4/91 infectious bronchitis live vaccines (IBLVs) and groups 5 and 6 received B1 and LaSota Newcastle disease live vaccines (NDLVs), respectively. At the age of 20 days, all birds in the experimental groups except the negative control group (group 1), were inoculated intra-nasally with H9N2 AIV. After the inoculation, clinical signs, gross and microscopic lesions, and viral detection were examined. The results of this study revealed that clinical signs, gross and microscopic lesions were more severe in the AIV challenged groups which had been previously vaccinated with IB vaccines. In addition, AI viral RNA from tracheal and faecal samples in IB vaccinated birds were recovered at a higher rate. Moreover, in the 4/91 IB vaccinated group, the AI virus shedding period was longer than the other challenged groups. In conclusion, infectious bronchitis live vaccines (IBLVs) exacerbated the H9N2 AIV infection; also, 4/91 IBLV extended AI virus shedding period and increased the recovery rate of AI virus from feaces. However, the coinfection of Newcastle disease live vaccines (NDLVs) had no considerable adverse effects on AIV infection in broiler chickens.

The mast cell mediators and distribution of lung mast cells in rats are often discussed in experimental studies on pulmonary fibrotic and allergic processes associated with changes in numbers of these cells, but information on the normal distribution of metachromatic and tryptase-positive mast cells in the interalveolar septa is scarce. There are no data on the presence of ghrelin in lung mast cells as well as the age-specific features of localisation and the number of mast cells in the interalveolar septa in rats of different ages. Therefore, the purpose of the present study was to determine the distribution of metachromatic, tryptase-, and ghrelin-positive mast cells in the interalveolar septa in 20 day-, 3 month- and 1 year-old rats. Tissue sections stained with toluidine blue had been taken from the left lung to visualise metachromasia and immunohistochemical expression of tryptase and ghrelin. The results showed that the amount of metachromatic mast cells in the interalveolar septa was significantly lower than that of tryptase- and ghrelin-positive cells. This allowed suggesting that mast cells were permanent occupants of the rat lung parenchyma and, on the other hand, the expression of ghrelin in their granules was most likely related to the synthesis of this protein. Our study showed that immunohistochemical identification by tryptase expression was more accurate than toluidine blue staining.

The stomach of birds comprises three parts – glandular (proventriculus gastris), muscular (ventriculus gastris) and pyloric (pars pylorica gastris). Depending on the diet, these three parts are well differentiated in omnivorous, herbivorous, insectivorous and granivorous birds and slightly differentiated in fish- and meateaters. In fruit-eating, nectar-eating and testacivorous birds, the stomach has an intermediate structure between the two types. In some birds from the genus Tangara, the stomach is reduced to a diverticulum.

This study was conducted to diagnose Babesia caballi in one-humped camels in Nineveh governorate, Iraq, using microscopic examination and conventional polymerase chain reaction (PCR) and to evaluate the clinical, haematological and blood biochemical parameters in camels. A total of 62 camels from local breeds, 2-7 years old from both sexes, reared in Nineveh Governorate, were naturally infected with B. caballi and 12 clinically healthy camels were used as controls based on clinical and laboratory examination. Babesia caballi was diagnosed as pear-shaped organisms, existing mainly in pairs within the erythrocytes in blood smears stained by Giemsa, with mean parasitaemia rate 8.59±0.13%. Blood smears stained by acridine orange and conventional PCR confirmed B. caballi diagnosis. Infected camels were found to be suffering from partial or total loss of appetite, emaciation, pale mucous membranes, irregular heart beats, rough hair coat, digestive disturbances, haemoglobinuria and presence of ticks on various body parts. There was considerably elevated body temperature, respiratory and heart rates among infected camels in comparison with control groups. Haematological examinations indicated a statistically significant (P<0.05) decrease in red blood cell counts, haemoglobin and haematocrit with macrocytic hypochromic anaemia. Additionally, leukocytosis due to significant (P<0.05) rise in total leukocyte count and lymphocytes in infected camels in comparison with controls besides a significant (P<0.05) decrease in platelet count and fibrinogen were demonstrated. On the other hand, the mean platelet volume, platelet distribution width, clotting time, prothrombin time and activated partial thromboplastin time were significantly (P<0.05) elevated in infected camels vs control. Biochemical analysis revealed significant (P<0.05) rise in troponin I, creatine phosphokinase myocardial band and lactate dehydrogenase in infected camels compared to healthy animals. This study concluded that B. caballi infected camels might be endemic in Nineveh governorate, Iraq. Babesia caballi in camels causes clinical, haematological, and blood biochemical changes.

The aim of the study was to perform histological differentiation of dorsal and ventral musculature of fresh and frozen/thawed carps (Cyprinus carpio). Histological findings of muscle fibres (Myofibra striata) of fresh carps did not show any changes. Single freezing at –10 ºС resulted in extracellular gaps in the central part of some of fibres. After single freezing at –18 ºС, muscle fibres with cell destruction in the central part were identified while the periphery remained intact. Completely destructured and deformed areas of muscle fibres were demonstrated after single freezing at –27 ºС. Double freezing at –10 ºС resulted in shrinkage, extracellular gaps and fragmentation of fibres, while muscle fibres double-frozen at –18 ºС were impaired, degraded and with visible defects. The histological findings in carp muscle, double-frozen at –27 ºС comprised severely deformed muscle fibres with increased extracellular gaps from degraded muscle tissue. On the basis of findings, it could be concluded that double freezing of carps was not an appropriate method of storage and shelf-life extension.

In this study the individual profiles of seminal plasma proteins (SPP) in rams of three breeds – Merino, Pleven Blackhead and Ile de France - were analysed. The study was carried out with three rams at 3, 6 and 10 years of age, grown and fed under similar conditions. Eighteen ejaculates (6 ejaculates from each ram) were evaluated by Sperm Class Analyzer. The total SPP concentration was measured spectrophotometrically. The separation and characterisation of SPP was performed by HPLC and one dimensional polyacrylamide gel electrophoresis (SDS-PAGE). There were no significant differences between the characteristics of ejaculates and the main kinematic parameters of the sperm in the breeds studied. Chromatograms showed specific profiles with 9, 10 and 11 protein peaks for Merino, Pleven Blackhead and Ile de France breeds, respectively. The total SPP concentration was the highest in the Pleven Blackhead breed and the lowest in Ile de France breed. The major parts of SPP in the three breeds were identical. The seminal plasma of Merino breed contained proteins with molecular mass of 30.3 kDa, 15.7 kDa and 15.2 kDa that were not present in the other two breeds. In the Ile de France and Pleven Blackhead samples only, two proteins with molecular masses of 39.7 kDa and 21.1 kDa, were observed. In conclusion, the detection of specific proteins can be used as a biological marker for sheep breed identification.

Bovine tuberculosis is still a serious problem with major economic impact in many countries. The aim of study was to evaluate the diagnostic capabilities of the classical and some modern, rapid methods for isolation and identification of Mycobacterium bovis. In the period 2015-2018 from 29 outbreaks in 10 different regions of Bulgaria, 1193 lymph nodes from slaughtered cattle were examined by pathoanatomical, bacteriological, PCR and immunochromatographic methods. Of the 283 bacterial isolates, 263 were identified as M. bovis - member of the M. tuberculosis complex.

The appearance of bacterial resistance to disinfectants and antiseptics is an issue of substantial health concern, resulting in low efficiency of epidemic control activities and emergence of microorganisms with cross-resistance to antibiotics and biocides. A synopsis of the main mechanisms of development of resistance to biocides is presented. The emphasis is placed to health risks and impact on medical practice. The main methods for detection of resistance, and prevention measures of key importance for its control are outlined.